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L2 Diagnostics

Representative publications from L2 research

Here is a selection of our peer-reviewed publications in vaccine, therapeutic, and diagnostic research, underpinned by our discoveries in structural, cell, and molecular biology.

Vaccines

AgBR1 and NeSt1 antisera protect mice from Aedes aegypti-borne Zika infection
A. Marin-Lopez, Y. Wang, J. Jiang, M. Ledizet, E. Fikrig
Vaccine, 2021

Fractionation of tick saliva reveals proteins associated with the development of acquired resistance to Ixodes scapularis
Jiří Černý, Geoffrey Lynn, Kathleen DePonte, Michel Ledizet, Sukanya Narasimhan, Erol Fikrig
Vaccine, 2020

Epidermal growth factor receptor peptide vaccination induces cross-reactive immunity to human EGFR, HER2, and HER3
Hester A Doyle, Raymond A Koski, Nathalie Bonafé, Ross A Bruck, Stephanie M Tagliatela, Renelle J Gee, Mark J Mamula
Cancer Immunology Immunotherapy, 2018

Vaccination with Aedes aegypti AgBR1 Delays Lethal Mosquito-Borne Zika Virus Infection in Mice
Yuchen Wang, Alejandro Marin-Lopez, Junjun Jiang, Michel Ledizet, Erol Fikrig
Vaccines, 2020

Inflammasome-activating nanoparticles as modular systems for optimizing vaccine efficacy
Stacey L Demento, Stephanie C Eisenbarth, Harald G Foellmer, Craig Platt, Michael J Caplan, W Mark Saltzman, Ira Mellman, Michel Ledizet, Erol Fikrig, Richard A Flavell, Tarek M Fahmy
Vaccine, 2009

Fusion loop peptide of the West Nile virus envelope protein is essential for pathogenesis and is recognized by a therapeutic cross-reactive human monoclonal antibody
Hameeda Sultana, Harald G. Foellmer, Girish Neelakanta, Theodore Oliphant, Michael Engle, Michel Ledizet, Manoj N. Krishnan, Nathalie Bonafé, Karen G. Anthony, Wayne A. Marasco, Paul Kaplan, Ruth R. Montgomery, Michael S. Diamond, Raymond A. Koski and Erol Fikrig
Journal of Immunology, 2009

A recombinant West Nile virus envelope protein vaccine candidate produced in Spodoptera frugiperda expresSF+ cells
Nathalie Bonafé, Joseph A. Rininger, Richard G. Chubet, Harald G. Foellmer, Stacey Fader, John F. Anderson, Sandra L. Bushmich, Karen Anthony, Michel Ledizet, Erol Fikrig, Raymond A. Koski, Paul Kaplan
Vaccine, 2009

Antibodies targeting linear determinants of the envelope protein protect mice against West Nile virus
Michel Ledizet, Kalipada Kar, Harald G Foellmer, Nathalie Bonafé, Karen G Anthony, L Hannah Gould, Sandra L Bushmich, Erol Fikrig, Raymond A Koski
Journal of Infectious Disease, 2007

A recombinant envelope protein vaccine against West Nile virus
Michel Ledizet, Kalipada Kar, Harald G Foellmer, Tian Wang, Sandra L Bushmich, John F Anderson, Erol Fikrig, Raymond A Koski
Vaccine, 2005

Protective and therapeutic capacity of human single-chain Fv-Fc fusion proteins against West Nile virus
L Hannah Gould, Jianhua Sui, Harald Foellmer, Theodore Oliphant, Tian Wang, Michel Ledizet, Akikazu Murakami, Kristin Noonan, Cassandra Lambeth, Kalipada Kar, John F Anderson, Aravinda M de Silva, Michael S Diamond, Raymond A Koski, Wayne A Marasco, Erol Fikrig
Journal of Virology, 2005

Jump to: Therapeutics | Diagnostics | Structural, Cell, and Molecular Biology


Therapeutics

Identification of Efflux Substrates Using a Riboswitch-Based Reporter in Pseudomonas aeruginosa
V. Urdaneta-Páez, R. Hamchand, K. Anthony, J. Crawford, A.G. Sutherland, B.I. Kazmierczak
mSphere. 2023

Screening for small molecule inhibitors of SAH nucleosidase using an SAH riboswitch
H. Sadeeshkumar, A. Balaji, A.G. Sutherland, S. Mootien, K.G. Anthony, R.R. Breaker
Analytical Biochemistry, 2023

Inhibition of Macrophage Migration Inhibitory Factor by a Chimera of Two Allosteric Binders
Pier F Cirillo, Oluwatoyin A Asojo, Uday Khire, Yashang Lee, Sara Mootien, Peter Hegan, Alan G Sutherland, Elizabeth Peterson-Roth, Michel Ledizet, Raymond A Koski, Karen G Anthony
ACS Medicinal Chemistry Letters, 2019

Coordination of different ligands to copper(II) and cobalt(III) metal centers enhances Zika virus and dengue virus loads in both arthropod cells and human keratinocytes
Shovan Dutta, Michael J Celestine, Supreet Khanal, Alexis Huddleston, Colin Simms, Jessa Faye Arca, Amlan Mitra, Loree Heller, Piotr J Kraj, Michel Ledizet, John F Anderson, Girish Neelakanta, Alvin A Holder, Hameeda Sultana
Biochimica et Biophysica Acta, 2018

Inhibition of mycobacterial alanine racemase activity and growth by thiadiazolidinones
Yashang Lee, Sara Mootien, Carolyn Shoen, Michelle Destefano, Pier Cirillo, Oluwatoyin A Asojo, Kacheong R Yeung, Michel Ledizet, Michael H Cynamon, Paul A Aristoff, Raymond A Koski, Paul A Kaplan, Karen G Anthony
Biochemical Pharmacology, 2013

Thiadiazolidinones: a new class of alanine racemase inhibitors with antimicrobial activity against methicillin-resistant Staphylococcus aureus
Mihai Ciustea, Sara Mootien, Adriana E Rosato, Oriana Perez, Pier Cirillo, Kacheong R Yeung, Michel Ledizet, Michael H Cynamon, Paul A Aristoff, Raymond A Koski, Paul A Kaplan, Karen G Anthony
Biochemical Pharmacology, 2012

A novel allosteric inhibitor of macrophage migration inhibitory factor (MIF)
Fengwei Bai, Oluwatoyin A Asojo, Pier Cirillo, Mihai Ciustea, Michel Ledizet, Paul A Aristoff, Lin Leng, Raymond A Koski, Thomas J Powell, Richard Bucala, Karen G Anthony
Journal of Biological Chemistry, 2012

New classes of alanine racemase inhibitors identified by high-throughput screening show antimicrobial activity against Mycobacterium tuberculosis
Karen G Anthony, Ulrich Strych, Kacheong R Yeung, Carolyn S Shoen, Oriana Perez, Kurt L Krause, Michael H Cynamon, Paul A Aristoff, Raymond A Koski
PLoS One, 2011

Effective siRNA targeting of the 3' untranslated region of the West Nile virus genome
Karen G Anthony, Fengwei Bai, Manoj N Krishnan, Erol Fikrig, Raymond A Koski
Antiviral Research, 2009

Antiviral peptides targeting the west nile virus envelope protein
Fengwei Bai, Terrence Town, Deepti Pradhan, Jonathan Cox, Ashish, Michel Ledizet, John F Anderson, Richard A Flavell, Joanna K Krueger, Raymond A Koski, Erol Fikrig
Journal of Virology, 2007

Discovery and pre-clinical development of antithrombotics from hematophagous invertebrates
Michel Ledizet, Lisa M Harrison, Raymond A Koski, Michael Cappello
Current Medicinal Chemistry, Cardiovascular Hematology Agents. 2005

Jump to: Vaccines | Diagnostics | Structural, Cell, and Molecular Biology


Diagnostics

Specific and Sensitive Diagnosis of Babesia microti Active Infection Using Monoclonal Antibodies to the Immunodominant Antigen BmGPI12
J. Gagnon, S. Timalsina, S. Mootien, C. Ben Mamoun, M. Ledizet et al
Journal of Clinical Microbiology, 2022

Evidence for SARS-CoV-2 Spike Protein in the Urine of COVID-19 Patients
J. Gagnon, S. Timalsina, M. Ledizet, the Yale IMPACT Team. et al
Kidney360, 2021

Circulating Unmethylated Insulin DNA As a Biomarker of Human Beta Cell Death: A Multi-laboratory Assay Comparison
Cate Speake ... Sahar Usmani-Brown, Carmella Evans-Molina et al
Journal of Clinical Endocrinology and Metabolism, 2020

Frequency and Geographic Distribution of Borrelia miyamotoi, Borrelia burgdorferi, and Babesia microti Infections in New England Residents
M. Ledizet, N. Lavoie, P.J. Krause et al
Clinical Infectious Disease, 2022

Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microti.
J. Gagnon, S. Timalsina, C. Ben Mamoun, M. Ledizet et al
Frontiers in Cellular and Infection Microbiology, 2022

BmGPAC, an Antigen Capture Assay for Detection of Active Babesia microti Infection
Jose Thekkiniath, Sara Mootien, Lauren Lawres, Benjamin A Perrin, Meital Gewirtz, Peter J Krause, Scott Williams, J Stone Doggett, Michel Ledizet, Choukri Ben Mamoun
Journal of Clinical Microbiology, 2018

Borrelia miyamotoi sensu lato seroreactivity and seroprevalence in the northeastern United States
Peter J Krause ... Sahar Usmani-Brown, Durland Fish, et al
Emerging Infectious Diseases, 2014

β cell responses to inflammation
Sahar Usmani-Brown, Ana Luisa Perdigoto, Nathalie Lavoie, Pamela Clark, Maria Korah, Jinxiu Rui, Gabriel Betancur, Kevan C Herold
Molecular Metabolism, 2020

Survey of Radiation Oncologists to Assess Interest and Potential Use of a Genetic Test Predicting Susceptibility for the Development of Toxicities After Prostate Cancer Radiation Therapy
Kayla Collado, Sarah L Kerns, Michael A Diefenbach, Elizabeth Peterson-Roth, Raymond Koski, Harry Ostrer, Richard G Stock, Martin Mattessich, Paul Kaplan, Barry S Rosenstein
Advances in Radiation Oncology, 2020

Direct Diagnostic Tests for Lyme Disease
Steven E. Schutzer ... Michel Ledizet, John A. Branda, et al
Clinical Infectious Disease, 2019

Unmethylated Insulin as an Adjunctive Marker of Beta Cell Death and Progression to Type 1 Diabetes in Participants at Risk for Diabetes
Kimber M Simmons ... Sahar Usmani-Brown et al, Type 1 Diabetes TrialNet Study Group
International Journal of Molecular Science, 2019

Advances in Serodiagnostic Testing for Lyme Disease Are at Hand
John A Branda ... Erol Fikrig, Michel Ledizet, Steven E Schutzer et al
Clinical Infectious Diseases, 2018

Development of Zika Virus Serological Testing Strategies in New York State
William T Lee ... Michel Ledizet, Ronald J Limberger et al
Journal of Clinical Microbiology, 2018

Unmethylated Insulin DNA Is Elevated After Total Pancreatectomy With Islet Autotransplantation: Assessment of a Novel Beta Cell Marker
M D Bellin, P. Clark, S Usmani-Brown, K C Herold et al
American Journal of Transplantation, 2017

β cell death and dysfunction during type 1 diabetes development in at-risk individuals
Kevan C Herold, Sahar Usmani-Brown, Tara Ghazi, Jasmin Lebastchi, Craig A Beam, Melena D Bellin, Michel Ledizet, Jay M Sosenko, Jeffrey P Krischer, Jerry P Palmer
Journal of Clinical Investigation, 2015

Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA
Brian T Backstedt, Ozlem Buyuktanir, Janet Lindow, Elsio A Wunder Jr., Mitermayer G Reis, Sahar Usmani-Brown, Michel Ledizet, Albert Ko, Utpal Pal
PLoS One, 2015

Analysis of β-cell death in type 1 diabetes by droplet digital PCR
Sahar Usmani-Brown, Jasmin Lebastchi, Andrea K Steck, Craig Beam, Kevan C Herold, Michel Ledizet
Endocrinology, 2014

The ability of virulence factor expression by Pseudomonas aeruginosa to predict clinical disease in hospitalized patients
Michel Ledizet, Thomas S Murray, Sailaja Puttagunta, Martin D Slade, Vincent J Quagliarello, Barbara I Kazmierczak
PLoS One. 2012

TLR9-targeted biodegradable nanoparticles as immunization vectors protect against West Nile encephalitis
Stacey L Demento, Nathalie Bonafé, Weiguo Cui, Susan M Kaech, Michael J Caplan, Erol Fikrig, Michel Ledizet, Tarek M Fahmy
Journal of Immunology, 2010

Serum antibodies to West Nile virus in naturally exposed and vaccinated horses
Louis A Magnarelli, Sandra L Bushmich, John F Anderson, Michel Ledizet, Raymond A Koski
Journal of Medicinal Microbiology, 2008

Detection of antibodies to West Nile virus in equine sera using microsphere immunoassay
Udeni B R Balasuriya, Pei-Yong Shi, Susan J Wong, Valerie L Demarest, Ian A Gardner, Pamela J Hullinger, Gregory L Ferraro, Joshua D Boone, Casey L De Cino, Amy L Glaser, Randall W Renshaw, Michel Ledizet, Raymond A Koski, N James MacLachlan
Journal of Veterinary Diagnostic Investigation, 2006

An indirect enzyme-linked immunosorbent assay for rapid and quantitative assessment of Type III virulence phenotypes of Pseudomonas aeruginosa isolates
Li Li, Michel Ledizet, Kalipada Kar, Raymond A Koski, Barbara I Kazmierczak
Annals of Clinical Microbiology and Antimicrobials, 2005

Detection of human anti-flavivirus antibodies with a west nile virus recombinant antigen microsphere immunoassay
Susan J Wong, Valerie L Demarest, Rebekah H Boyle, Tian Wang, Michel Ledizet, Kalipada Kar, Laura D Kramer, Erol Fikrig, Raymond A Koski
Journal of Clinical Microbiology, 2004

Jump to: Vaccines | Therapeutics | Structural, Cell, and Molecular Biology


Structural, Cell, and Molecular Biology

Methylation of insulin DNA in response to proinflammatory cytokines during the progression of autoimmune diabetes in NOD mice
Jinxiu Rui, Songyan Deng, Jasmin Lebastchi, Pamela L Clark, Sahar Usmani-Brown, Kevan C Herold
Diabetologia, 2016

Dengue Virus Infection of Aedes aegypti Requires a Putative Cysteine Rich Venom Protein
Berlin Londono-Renteria ... Diana Vesely, Michael Ledizet, Tonya M Colpitts et al
PLoS Pathogens, 2015

Structural and biochemical analyses of alanine racemase from the multidrug-resistant Clostridium difficile strain 630
Oluwatoyin A Asojo, Sara Mootien, Yashang Lee, Wanderson C Rezende, Daniel A Hyman, Monica M Matsumoto, Scott Reiling, Alan Kelleher, Michel Ledizet, Raymond A Koski, Karen G Anthony
Acta Crystallographica D Biological Crystallography, 2014

Evidence for vesicle-mediated antigen export by the human pathogen Babesia microti
Jose Thekkiniath, Nicole Kilian, Lauren Lawres, Meital A Gewirtz, Morven M Graham, Xinran Liu, Michel Ledizet, Choukri Ben Mamoun
Life Science Alliance, 2019

Characterization of Ixophilin, a thrombin inhibitor from the gut of Ixodes scapularis
Sukanya Narasimhan, Oriana Perez, Sara Mootien, Kathleen DePonte, Raymond A Koski, Erol Fikrig, Michel Ledizet
PLoS One. 2013

Structural studies of human glioma pathogenesis-related protein 1
O. A. Asojo, R. A. Koski and N. Bonafé
Acta Crystallographica D Biological Crystallography, 2011

Expression, purification, crystallization and preliminary X-ray analysis of a truncated soluble domain of human glioma pathogenesis-related protein 1
Nathalie Bonafé, Bin Zhan, Maria Elena Bottazzi, Oriana A Perez, Raymond A Koski, Oluwatoyin A Asojo
Acta Crystallographica F Structural Biology Crystallographic Communications, 2010

Swarming motility, secretion of type 3 effectors and biofilm formation phenotypes exhibited within a large cohort of Pseudomonas aeruginosa clinical isolates
Thomas S Murray, Michel Ledizet, Barbara I Kazmierczak
Journal of Medicinal Microbiology, 2010

Crystal structure of dengue virus type 1 envelope protein in the postfusion conformation and its implications for membrane fusion
Vinod Nayak, Moshe Dessau, Kaury Kucera, Karen Anthony, Michel Ledizet, Yorgo Modis
Journal of Virology, 2009

West Nile virus envelope protein inhibits dsRNA-induced innate immune responses
Alvaro Arjona, Michel Ledizet, Karen Anthony, Nathalie Bonafé, Yorgo Modis, Terrence Town, Erol Fikrig
Journal of Immunology, 2007

Crystal structure of West Nile virus envelope glycoprotein reveals viral surface epitopes
Ryuta Kanai, Kalipada Kar, Karen Anthony, L. Hannah Gould, Michel Ledizet, Erol Fikrig, Wayne A. Marasco, Raymond A. Koski, Yorgo Modis
Journal of Virology, 2006

Isoaspartyl post-translational modification triggers anti-tumor T and B lymphocyte immunity
Hester A Doyle, Jing Zhou, Martin J Wolff, Bohdan P Harvey, Robert M Roman, Renelle J Gee, Raymond A Koski, Mark J Mamula
Journal of Biological Chemistry, 2006

Gamma delta T cells facilitate adaptive immunity against West Nile virus infection in mice
Tian Wang, Yunfei Gao, Eileen Scully, C Todd Davis, John F Anderson, Thomas Welte, Michel Ledizet, Raymond Koski, Joseph A Madri, Alan Barrett, Zhinan Yin, Joseph Craft, Erol Fikrig
Journal of Immunology, 2006

Jump to: Vaccines | Diagnostics | Therapeutics